Recombinant Human CTLA4 Protein (Fc & Avi Tag)(Active)

Sizes: 20μg, 100μg

Catalogue Numbers: PKSH033803-20, PKSH033803-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CTLA4

Target Symptom: Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA4; CD152; Cytotoxic T-Lymphocyte-Associated Protein 4

Research Area: immunology; Stem cells

Conjugation: Biotinylated

Target Species: Human

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-Fc-Avi

UNIProt ID: P16410

Accession: P16410

Background: Cytotoxic T-lymphocyte protein 4; is a single-pass type I membrane protein. It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

Activity: Immobilized Human CTLA-4-Fc-Avi at 2μg/ml(100 μl/well) can bind Human B7-2-6His(Cat: PKSH033722). The ED50 of Human CTLA-4-Fc-Avi is 1. 98 ug/mL

Sequence: Lys36-Asp161

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 41.5 kDa

Observed MW: 57-60 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only