Phospho-RB-S807/811 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79379-50, BS79379-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06400

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HT-29 cells, using Phospho-RB-S807/811 antibody at 1:1000 dilution. HT-29 cells were treated by Nocodazole for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 120kDa

Specificity: Phosphorylated

Alternative Name: OSRC; PPP1R130; RB; p105-Rb; pRb; pp110; RB1; Rb

Immunogen: A synthetic phosphorylated peptide around S807 & S811 of human RB(NP_000312.2).

Conjugate: Unconjugated

Modification: Phosphorylated