Recombinant Human BDNF Protein (with pro peptide)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033559-10, PKSH033559-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: BDNF

Target Symptom: Brain-Derived Neurotrophic Factor; BDNF; Abrineurin

Research Area: Cardiovascular; Neuroscience; metabolism

Target Species: Human

Expression Host: E.coli

Application: ELISA

Fusion Tag: None

UNIProt ID: P23560

Accession: P23560

Background: The precursor form of Brain-Derived Neurotrophic Factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis; whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival; growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects; Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Activity: Immobilized Human pro-BDNF at 2ug/ml(100 μl/well) can bind Human TrkB-His(Cat: PKSH033579). The ED50 of Human pro-BDNF is 1. 4 ug/ml.

Sequence: Ala19-Arg247(R125A, R127A, R128A)

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM NaCl, 0.02% Tween 80, pH 6.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 25.6 kDa

Observed MW: 28 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only